Storyline 360 lets you create courses that support Web Content Accessibility Guidelines (WCAG).
Storyline 360 courses support WCAG 2.1 Level AA, including screen readers, keyboard navigation, visible focus indicators, and more. See this article for design recommendations.
Accessibility How-To Articles
See these articles to learn more about authoring accessible courses:
Screen Reader Support
Storyline 360 supports these screen readers with HTML5 and Flash courses: